Sentence examples for of the total template from inspiring English sources

Whole-genome amplification was performed on 10 ng of the total template DNA using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare) following the manufacturer's instructions.

Here we discuss how the three methods of (1) MDTS, (2) MLTS, and (3) template compression performed when using the LLR and KL distances and show the results in Figure 8, where the number of template representatives were kept to be 20% of the total templates for the three cases (further details are discussed in Section 5.3.5).

These data indicate that the proportion of templates amplified varied between 26 61% of the total templates available.

In Figure 9, we show how the percentages of template representatives selected from the total templates affect phone accuracies for MDTS and MLTS with their respectively compatible distances.

The first-strand cDNA was synthesized using 250 ng of the total RNA template using the PrimeScript RT Master Mix Perfect Real Time kit (Takara Co., Otsu, Japan).

The actin and GAPDH ratios indirectly reflect the efficiency of reverse transcription of the total RNA template, as high 3′ to 5′ ratios indicate poor transcription from the 3′-end, resulting in small fragments of cDNA/cRNA available for hybridization.

B. burgdorferi's small genome (~1.5 Mb), is overwhelmed by the genome size of its black-legged tick vector, Ixodes scapularis (~2.1Gb) [ 14– 17] and, in average field collected nymphal ticks, B. burgdorferi represents <0.01 % of the total DNA template.

The areas on the pain drawings were measured in square millimeters and calculated as percentage of the total body template area using a commercial software program (Quantify One; K L O N K, Sorø, Denmark), a method that has been shown to be reliable for quantifying pain drawings [ 51, 52].

The scores for templates utilizing methane PCI and EI are given by the following eqs 1 and 2: 1 2where I j and M j are the S/N and m/ z, respectively, of the jth member of n total template members.

The PCR reaction (50 μl) contained 5 ng of total template DNA (equimolar mixture of each parental gene), 0.3 μM of each primer, 0.2 mM of each dNTP, and 5 IU Taq polymerase.

The qPCR reactions were carried out in Hard-Shell semi skirted clear 96 well plates (Bio Rad) sealed with Microseal B film (Bio Rad) in 25 μl volumes using IQ SYBR green supermix (Bio-Rad) according to the manufacturer's instructions with 200 nM of forward and reverse primer and 12ng (sample A) or 5ng (sample B) of the total DNA as template.