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In non-auditory structures, pentose shunt flux was ∼7% of the total label recovered in metabolites of [6-C]glucose (i.e. trapped via the oxidative pathways), a value similar to the ∼2 8% of glucose metabolism obtained in previous studies in rat brain (Ben-Yoseph et al., 1995 and cited references).
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The hcDNA-HMGB1 complex formed, which may contain up to 25 30% of the total labeled DNA, is purified by electrophoresis on a 4% polyacrylamide gel (see below) and electroelution.
Labeled claudin-4 was present in the media as early as 2 hours post-labeling and reached a level of approximately 3 4% of the total labeled claudin-4 after 24 hours.
The radioactivity of the total labeled proteins was determined after a trichloroacetic acid precipitation.
Note: number represented the percentages (mean ± SD) of the total labeling in distinct locations in root-cap cells of Arabidopsis.
The numbers in table 1 represented the percentage (mean ± SD) of the total labeling in distinct locations in whole cytoplasm.
Labeling experiments with NH4+ in combination with NO3− showed that N2 was the dominant product, making up 98.2% of the total labeled N2.
The detected coincidence levels corresponded to 4.8 ± 1.1% of the total labeled molecules present being involved in complexes containing at least one YFP−dyskerin and one 647N−hTR component.
Such ions form a small fraction of the total labeled activity (due to much higher internal surface area in each MSN).
The contour curves were defined so that, for example, the 25% contour encloses the top 25% percentile of the total labelling from the kernel density estimate (Sterratt et al., 2013).
We assessed these changes using a laterality index LI, which is based on the proportion of the total labeling pattern that is contained in 4 equally spaced compartments oriented parallel to the lateral border of V1.
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