This significantly improved the measured biodegradation due to reduced abiotic losses and better retention of the test substance.
"It's wonderful," she said of the test substance, a weight loss serum under development by the Danish biotechnology giant Novo Nordisk.
Results indicated that abiotic losses of test substances were occurring due to sorption of the test substance to plastic components used in the OxiTop® system.
The test was then initiated by the application of the test substance, incubation, and temporal assessment of corneal damage using various parameters.
For this, the carbon content of the test substance needs to be determined.
Neither stability nor by-products of the test substance was stated in any of the studies.
The purity of the test substance was not stated in six of the nine studies.
They are then transferred to a medium free of the test substance for the depuration phase.
The modified method, with two-step addition of the test substance, yields the expected results.
In addition, the relevant physico-chemical data of the test substance (solubility in water, volatility, adsorptivity) that could possibly influence the meaningfulness of the test result should be known, as well as the purity of the test substance.
The organic carbon content of the test substance is determined as the TOC (g/g) (according to DIN EN 13137 in a double determination) and a known amount of the test substance is used in the test.
