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NGS strategies based on deep sequencing overcome some of the technical drawbacks of probe-based methodologies, especially the ability to detect only previously known sequences (Schulte et al. 2010).
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We anticipate that due to the technical drawbacks in identifying all relevant peroxisomal components the atlas of peroxisome functions is still far from being complete.
Significant variations in sensitivity and negative predictive values have been reported in the scientific literature, reflecting both the syndromic nature of the disease and the technical drawbacks of conventional CMR sequences, with evidence that a negative CMR exam cannot reliably rule out a diagnosis of AM.
Others have commented upon a sound delay as the main technical drawback of the instrument (if one would dare criticize the Renaissance Man or his craftsman at this time!).
DNA microarrays have been used to determine the complete transcriptomic profile of microorganisms in vitro, but technical drawbacks that are associated with the use of this technique in vivo, such as the qualitative and quantitative recovery of bacterial RNA from host tissues, explain why the use of this technique to monitor bacterial infections has been limited [ 21].
The biggest technical drawback of our solution is computational efficiency.
Even though the self-reported personal skill to manage PD complications is similar to HD, there are still some perceived barriers to PD such as the risk of encapsulating peritoneal sclerosis and/or technical drawbacks.
The clonogenic assay is a long term assay but technical drawbacks and the long culturing time have limited the clinical use of the method [ 9, 10].
The 26 markers with the highest quality score had no technical drawbacks, such as presence of stuttering or amplification of extra peaks.
Back in those days, however, technical drawbacks of early CT systems limited the application of dual- and multi-energy CT imaging in daily clinical routine [32].
Many respondents recognized the drawbacks of the technical assistance provided, characterized by high turnover and little coordination, which resulted in the loss of institutional memory, duplications and incoherence in policy-making and implementation.
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