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They argue there should just be more of the same: leave things as they are, but do it better.
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The HHV of gaseous products of different leaves, using the same pyrolysis treatment, showed similar values but noticeably different when the pyrolysis process is for the same leaves is changed.
Width was taken at the widest point (middle) of the same leaves.
Specific leaf weight, chlorophyll content, and nitrogen content of the same leaves used for gas exchange measurement were measured.
Infiltration of leaves with 600 µM CPZ solution 30 min prior to inoculation of the same leaves with TBSV virion preparation resulted in 99% inhibition of TBSV RNA accumulation at 4 dpi (Fig. 5A).
We also determined the soil and plant analyzer development (SPAD) values of the same leaves used for stomatal conductance measurements.
Interestingly, when the SlMKK2 DD or SlMKK4 DD construct was transiently expressed in one half of leaves, the opposite half of the same leaves showed enhanced resistance to B. cinerea and upregulated expression of defense genes upon infection of B. cinerea, indicating that SlMKK2 and SlMKK4 may have a systemic effect on activation of defense response.
For example, scans that have been used for the measurement of individual area of successive leaves on a rosette can be re-analysed to estimate shape parameters of the same leaves; similarly, leaf sections that have been used in the estimation of leaf thickness can be used in the measurement of vein diameter.
In our experiments, significant H2O2 accumulation due to transient expression of SlMKK2 DD or SlMKK4 DD construct in one half of the N. benthamiana leaves at 48 hr after infiltration, at the time when the opposite half of the same leaves was inoculated with B. cinerea, may mount the ROS generated during the early stage of infection.
Many of their descendants did the same, leaving their slave ancestry behind.
The logarithm of the FER showed a good agreement with the absorbance of methanolic extracts obtained from the same leaves.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com