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Trueness and precision also have different practical consequences: trueness is necessary in reaching targets, precision is necessary to identify rapid changes of the quantity value.
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For each gene, standardized quantity values were calculated by dividing the mean of the quantity values for the three technical replicates for each sample by the mean of the replicate values of the RCP1 exogenous control for that sample.
Measurement uncertainty is a parameter characterizing the dispersion of the quantity values being attributed to a measurand based on the information used.
However, these measurements need to be calibrated using the quantity value of a material having ideally the same chemical nature and molecular weight as the analyte of interest.
In medical practice, measurements usually have 3 main objectives: (1) diagnosis, by a timely comparison of the actual quantity value with its normal range, (2) alarm, by continuously checking if the quantity value is inside or outside a predetermined range, and (3) titration, by finely tuning a therapeutic action to bring a quantity value toward a targeted range.
The quantity values of newly matured oocytes collected 19 h of IVM were arbitrarily set as 100%, and the other values were expressed relative to this activity.
The quantity values of the spots were exported after detection, standardization, and background elimination.
Estimation of the quantity and value of felled material.
Samples amplified after cycle 35 were censored in accordance with the recommendations from Life Technologies, and the median quantity value of the triplicates was used for data analysis.
The obtained quantity values were standardised to mean one by division with the median quantity value of the 15 examined probes (the median value was considered to be most likely to represent a normal copy number).
Instead of using each fusion marker separately with reduced statistic power, we proposed to calculate the quantitative feature of combined fusion markers based on the sum of quantity values (or the highest relative quantity) of fusion markers in each sample.
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