Sentence examples for of the primary template from inspiring English sources

Structural sizes in 3D ZnO structures obtained through templating processes depend highly on the sizes of the primary template.

For second strand synthesis, PCR was carried out on a small aliquot (1/10th volume) of the primary template by using Advantage 2 Polymerase Mix (Clontech).

For second-strand synthesis, PCR was carried out using a small aliquot (1/10th volume) of the primary template and Advantage 2 Polymerase Mix (Clontech).

For the second strand synthesis, touch-down PCR was performed using a small aliquot (1/20 volume) of the primary template with EX Taq (Takara), the 5' PCR primer II A (Clontech) and a gene specific primer.

For the dopamine transporter example, we chose an amine transporter from Aquifex aeolicus VF5, identified by HHBlits with a sequence identity of ∼24%, as the primary template.

Even though this region of controversial connectivity is quite distant from the anesthetic binding site in question, another model of LyTASK was constructed utilizing the amino acid backbone connectivity corrected to that of 4I9W along with 3UKM as the primary template.

The second round was performed in a final volume of 25  μl with 2.5  μl of the primary PCR template as described above.

The size of the primary gene template encoded in the maxicircle is radically minimized, with up to half of a gene's coding information stored in gRNAs whose function is largely unaffected by transition mutations.

A total of 14 primer pairs amplified paralogous sequences, at a mean of 2.5 per primary template sequence, with a range from 1 12.

To amplify fragment 2100 bp-RACE, the primers were identical to those used to amplify Sequence C and the PCR conditions similar except that Advantage polymerase mix was used and the nested PCR was done using a 1 200 dilution of the primary PCR reaction as template.

While the industry has long claimed that one of the primary benefits of biometric templates is that original biometric signals acquired to enroll a data subject cannot be reconstructed from stored templates, several approaches [2, 3] have proven this claim wrong.