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The degree of crystallinity of the PHB and PHB-HV nanofiber samples in our study is given in Table 2, from which it can be seen that the PHB-HV12 sample had the lowest degree of crystallinity.
The molecular weight of the PHB and PHB-HV samples is shown in Table 2.
Kinetic analysis of PHB degradation suggests that this amino acid substitution promoted not only the adsorption of the mutant enzyme to PHB, but also the disruption of the PHB surface to enhance the hydrolysis of the PHB polymer chain.
Furthermore, the phaZ gene that encodes a PHB depolymerase (Additional file 1), the first enzyme of the PHB degradation pathway, was also found in its genome.
The X-ray diffraction patterns of the PHB in the nanocomposite film showed peaks corresponding to the crystallized PHB.
Benefits for the PHB production are likely to be found in the growth of the bacterial organisms instead of the PHB metabolism.
PhaZ2 was also shown to play a role in PHB degradation ([York et al. 2003]), suggesting its presence on the surface of the PHB granule.
The parts marked in blue are the components of the PHB metabolic pathway.
Our results also point towards the importance of aeration on the synthesis of the PHB pathway precursor and cofactors.
The recovery of the PHB produced was optimised and the isolated polymer characterised to identify its material properties.
The assessment of the PHB criterion is carried through after the marketing authorization process and is an addition to it.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com