The appearance of the nucleus texture is related to the distribution of the chromatin within the nucleus membrane.
The mean value feature is a measure of the nucleus texture intensity and it is related to the existence of P63 receptors in the nucleus.
This may be explained by considering the destruction of P63 receptors in high grade nuclei, which contributes to randomness in the location of the remaining P63 receptors within the nucleus texture, thus resulting in increased variations in the staining of the nucleus texture across the nucleus image.
To add randomness to the nucleus texture generation, each new texture is generated from three different sample nuclei randomly selected from the database.
Therefore, the final image, I f x, y) is updated according to (15) where is a feathered nucleus mask generated as (16) The feathering, or blurring, of the nucleus mask,, prevents the creation of sharp, unnatural edges around the nucleus texture.
The edge statistics feature, which is the eighth component of the eight bin magnitude histogram, echoes the frequency of high intensity pixels in the nucleus texture.
Thus, the question arises if there might exist a correlation between the nucleus texture of P63-expressed nuclei and the tumor's grade, as determined by H&E staining.
Because the nucleus texture is a product of patch-based texture generation from source material, the texture has actually already been subjected to the degenerative effects of the optics and the sensor system.
MN characteristics are rounded smooth perimeter suggestive of a membrane, less than a third the diameter of the associated nucleus, but large enough to discern shape and color; staining intensity similar to that of the nucleus; similar texture to that of nucleus; same focal plane as nucleus; and absence of overlap with, or bridge to, the nucleus.
The generation of the phantom image can then be written as a sum of phantom objects with appropriate amounts of out-of-focus blur, (14) The final step of the synthesis process is to remove some of the degenerative effects applied to the parts of the image displaying nucleus texture.
Most probably, the loss of continuum in stained nucleus texture, due to the destruction of P63 receptors at advance histological grades, results in the formation of a multitude of microstructures of various sizes and, thus, in a nucleus texture of increased inhomogeneous appearance and loss of image linearity.
