Sentence examples for of the initial template from inspiring English sources
Quantification of the initial template copy number is possible by monitoring fluorescence at each cycle at a constant temperature.
The time (Tt) required for the turbidity of the LAMP reaction solution to exceed a given value was dependent on the quantity of the initial template DNA.
SPR data also showed that vesicle immobilization could be achieved through an integrin integrin antibody interaction after activation of the initial template with a goat anti-human integrin β1 antibody.
Activation of the initial template is effected by sequential immobilization of avidin, which binds to the biotin in the initial template, a biotinylated goat anti-mouse immunoglobulin G (Bt-IgG), which binds to the avidin binding sites distal to the surface and the Fc portion of the rho 1D4 antibody through its Fab region(s) and finally rho 1D4.
We present a method for creating highly ordered tissue-only constructs by using rigid microtopographically patterned surfaces to first guide myoblast alignment, followed by transfer of aligned myotubes into a degradable hydrogel and self-organization of the ordered cells into a functional, 3-dimensional, free-standing construct independent of the initial template substrate.
By overgrowing these holes with different amounts of Al0.33Ga0.67As, the shape and size of the initial template can be tuned.
Thus, at least in vitro, under certain transcription conditions, there is no commitment of any limiting transcription factor to the initial template to form a pre-licensed reinitiation scaffold (Kadonaga, 1990).
The interview guide and findings presented in a previous study [ 11] served as basis for the initial template consisting of two higher-order themes: 1) "Strenuous work situation for HCWs" and 2) "Economy"; themes initially indicated to be characterized by inter-level discrepancies.
This was both to control the mutation rate by ensuring that the reaction did not saturate the reagents, which would cause the number of doublings to become sensitive to the initial template concentration, and to avoid the PCR-based recombination events that can occur during with the last few thermal cycles of PCR reactions [ 38, 39].
(18) Although the last two methods are capable of reusing the initial template, deposition is limited to single materials and requires multiple steps for making detailed patterns.
We calculated the two-dimensional self-correlation and fast-Fourier transform (FFT) of the AFM images of the initial templates, in order to characterize the pore order and interdistance.
