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This finding correlates well with the results of the qRT-PCR analysis, showing that 20%% (sFGFP) and 49%% (mCherry) of the initial copies of the spike were present after 144 h of storage.
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The first scenario, as illustrated in Figure 4A, proposes that the initial copy of gene a (i.e., a.1) inserted before the divergence of the AA and BB genome, and the initial copy of gene b (i.e., b.1) inserted only in the AA species after the AA and BB species divergence.
Data are expressed as mean (SD) of the logarithm of the initial copy number.
RainDance and BioRad offer extremely high throughput digital PCR machines utilizing millions of droplets to enable quantification of the initial copy number of the target nucleic acids, but the process takes well over an hour and requires a large table-top system for droplet generation and interrogation.
E (m, t ) is thus intuitively interpretable as a product of the initial copy number with the effects of halving at each cell division, and the copy number evolution through past and current cell cycles and quiescent phases.
Of course, it is also possible that the insertions of initial copies of genes a and b and the subsequent duplication of the gene cluster (a.1, 12.2 and b.1) occurred before the divergence of the AA and BB species.
We observed a significant correlation between the number of initial copies of each IS type and the number of events observed after 80 thinsp;500 generations (figure 1; Spearman's coefficient = 0.78, p = 0.022).
The mean Ct and PCR efficiency values were used to estimate the initial copy number (ICN) of mRNA transcripts of each particular gene, including the house-keeping gene (B2M) [16].
The mean Ct and PCR efficiency values were used to estimate the initial copy number (ICN) of mRNA transcripts of each particular gene, including the house-keeping gene (B2M) [ 16].
Initial copies of the soundtrack also came with a special "Wind-up Bahamut" in-game pet.
The level of gene expression was quantified using the initial copy number.
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