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The enzymatic GUS activity was calculated on the base of the O.D. recorded and the molar extinction coefficient of the GUS substrate p-nitrophenol.
They were members of the Gus Giordano Dancers, the famous Chicago-based jazz-dance group.
Currently she is the other woman in the life of the Gus Eisman who sends her off to Europe for some cultivation.
David Podsiadlo, inset, of the Gus design group in Toronto, designed the Douglas Return Bench, made of a plank of Canadian Douglas fir and stainless steel legs.
Plants were photographed to record deposition of the GUS.
The expression of the GUS gene was confirmed by histochemistry and polymerase chain reaction.
Percent transgenic seed at each location was determined by testing the seedlings for the presence of the GUS marker gene.
The maximum accumulation of the GUS protein was observed in the inner starchy endosperm, close to the late maturation stage.
Fluorometric analysis of the GUS activity revealed a 4-fold4-foldase in the activity of the full-length Des promoter compared to the CaMV35S promoter.
For detailed observation of the GUS expression pattern in transgenic cells, explants were sectioned using a vibratome according to the protocol mentioned in the previous section.
The results of the GUS reporter gene assay with X-Gluc staining indicated that SFAR4 is highly expressed during seed germination and early seedling growth (Fig. 2a d).
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