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Proteins that were identified in the isotype control immunoprecipitations were filtered out of the final interpretation.
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The right sampling of homologous sequences for phylogenetic or molecular evolution analyses is a crucial step, the quality of which can have a significant impact on the final interpretation of the study.
The discussion rounds were stopped once the research team achieved agreement on the final interpretation of the data analysis.
Discordance about study inclusion between the two reviewers was resolved through discussion until 100% agreement was reached on the final interpretation of the data.
Multiple biological or analyte-related factors, assay specifications, study parameters and the context in which the results are interpreted are often under-estimated and ignored in the study design phase but can have tremendous impact on the final interpretation of the results.
Therefore, an accurate knowledge of the chosen animal model is continuously useful from the conception of a study to the final interpretation of the results obtained.
TM was responsible for the examination of the blood samples and for the final interpretation of the obtained data.
AL and SM advised for the search of neuropsychological consequences in critical patients and contributed to the final interpretation of these consequences on the final manuscript.
Figure 1 illustrates the steps of a non-targeted clinical metabolomics study from the preanalytical phase to the final interpretation of the data: Design the experiment (see section " Preparation before blood collection ").
Several limitations should be considered for the final interpretation of results.
Comparison of the Panbio unit values and of final interpretations (positive or negative using manufacturer's criteria) for all ELISAs (Table 1) demonstrated no significant differences, with the exception of the JEV/Dengue Combo IgM ELISA, which showed significantly different results for plasma and serum for the Panbio unit comparison (P = 0.02) but not for the final interpretation (P = 0.5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com