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Different species have codon usage bias, and optimizing the genetic codon according to the bias of the expressing host could increase the expression efficiency of the target gene [ 34- 36].
The expression of the target proteins using the same vectors in vitro showed that differences in expression level were wholly dependent on the environment of the expressing cell and an investigation of the time course of expression showed it could affect substantially the observed expression level for poorly but not well-expressed proteins.
Thus, the initiation of expression in one cell allows the spread of the expressing entity to other cells.
A 3 x influenza HA epitope tag was inserted into the expression vectors at the position corresponding to the C terminus of the expressing protein.
One of the 13 clones was sorted for reporter expression, and the FISH analysis revealed that in >50% of the expressing cells, the reporter construct was present exclusively at the originally tagged telomere.
In regard to expression, previous studies were either based mainly on the location of the expressing cells in relation to the VZ or did not examine different AP levels (Delaloy et al., 2010; Deo et al., 2006; Leucht et al., 2008; Shibata et al., 2008).
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Activity of the expressed protein was 0.77 μmol min−1.
Majority of the expressed protein was observed in inclusion bodies.
As expected, neutral is the majority of the expressed emotions.
High level expressed HPT was achieved but most of the expressed protein aggregated as inclusion bodies.
A significant amount of the expressed streptokinase was found in the soluble form in the cytoplasm.
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