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"I've always been fond of the 'Did Dad die in Baghdad?' sketch, which goes back to Dan Leno's pantomimes at Drury Lane: it's just a stream of ad-libbed nonsense.
There are undeniable pleasures to be gleaned from watching Vincent Gallo perform both the De Niro and the Pesci parts of the "Did you fuck my wife?" exchange from Raging Bull, in front of a cinema screen showing the actual scene.
Then, what the NSA can do is it can query that database to see did any of the — did this number pop up?
But alas, only one of the "did not happens" was lamented.
CeA knockdown of Homer2b decreases total alcohol intake and intake of 40% alcohol in a four-bottle-choice version of the DID procedure.
We performed a modified version of the DID protocol (Supplementary Figure 1C) in which mice had access to either 5% sucrose solution (days 1 9) and water (days 10 15), or alternatively 1% sucrose solution only (days 1–9).
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Some of the did-not-happens were not so happy.
Notably, the absence of the CC region does not affect the oligomerization state of the DID-DD fragment (Fig. S1) and G-DID-DD-CC and DID-DD inhibit FH2-DAD with equal potency in in vitro actin assembly assays (Fig. S2).
The 1H-15N TROSY-HSQC spectra of 250 µM 2H/15N-labeled DID complexed with unlabeled DAD in the absence and presence of unlabeled DAD-less FH2 (250 µM) shows no peak shifts or broadening (Fig. S7), indicating that there is no interaction between DID and bridge at this concentration range (or the population of the DID-bridge complex is too small to detect by this method).
There were rumors, yes, of the did-she-or-didn't-she variety.
The structure exhibits a tetrameric configuration that contains four chains each of the DID-DD and FH2-DAD.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com