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The growth rate of the complementing strains was evaluated in the absence of DAP.
However, DS cultures of the complementing strain IH101 (MRS123) did show Ssp4 expression, thereby demonstrating that Spo0A is specifically required for Ssp4 production.
Three of the complementing dpolv mutants in exon 11 are missense mutations but the complementing mutant dpolv105A is due to a nonsense mutation, and dpolv6 in exon 15 on the other side of the PIGSFEAST exon is a frameshift mutation which generates a stop codon.
Because overproduction of AsdA enzyme increases generation times [19], [34] and synthesis of Asd enzyme is proportional to the copy number of the complementing plasmid, asdA mutants were complemented with E. ictaluri asdA gene with (PasdA-asdA) and without its promoter (SD-asdA), this last to decrease Asd synthesis, cloned into p15A ori plasmid (pACYC184; Table 2).
Presence of the complementing construct in each line was confirmed by PCR on genomic DNA.
To bring about overexpression of the complementing gene, promoter-less copies were cloned into pMTL-ME6X downstream of the strong Pfdx promoter.
Similar(51)
By mixing more or less of the complement, you can lower the saturation a lot or a little.
Instead of the complement of two biological parents seen in natural reproduction or in I.V.F., cloned cells -- or babies -- are exact replicas of one individual.
Both pathways converge to activate the pivotal protein of the complement system, called C3.
The bactericidal efficiency of the complement pathway was further determined.
Furthermore, IgMs are potent activators of the complement system.
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