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To determine the role of the alignment method in generating strand bias, we aligned our samples using an additional aligner: Bowtie [ 13].
On aligned scaffolds of large fibers (∼740 nm) cell directionality closely follows the underlying fibers, irrespective of the alignment method.
The result of experiment has demonstrated the efficiency and automatic characteristic of the alignment method compared with other two convention methods.
In addition, the accuracy of the alignment method varies depending on the numerical solutions adopted to efficiently perform exhaustive searches for three orientation parameters.
This strategy is proved to be viable and the validation indicates that quantitative result obtained using this strategy mainly depends on the efficiency of the alignment method as well as chromatographic peak shape of the chromatograms.
By using data gathered from a previous study, we were able to repeatedly align a molecule's attachment sites via simulation of the program, thereby demonstrating the effectiveness of the alignment method.
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When evaluated in terms of speed, the alignment method described above was run with a straight ProbconsRNA implementation.
The aim of the study is the research of the alignment methods applied for the rotating kilns, their merits and disadvantages.
It is demonstrated that the efficiency of the alignment methods can be improved if approximate centers of gravity of particle views are known.
Alignments are clearly informative about error but their use has certain drawbacks: they cannot be used uniformly across the assembly (because some regions do not align), they are not equally informative about all species (because of differences in phylogenetic position), and their usefulness depends on the accuracy of the alignment methods.
On the other hand, the scoring system of the alignment methods is far from perfect.
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