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Exact(16)
For instance, the macroscopic size of the alignment length allows the light diffraction (results not shown).
We found that 99% of these alignments meet follow criteria: (1) the number of consensus positions is ≥15 or ≥15% of the alignment length, and (2) consensus residues must contain ≥2 active residues (E, D, R, K, H, S, T, C, N, and Q).
With a chosen threshold of the alignment length (bps) and identity, a list of putative protein functions was created.
Each alignment has fewer than 50 middle gaps, and the number of terminal gaps is <20% of the alignment length.
Reads were mapped only if the pairwise sequence identity with a reference isotig was at least 98% of the alignment length.
When the overlapping cut off was set to the 30% of the alignment length, virtually no hits were recovered for the unassembled reads (Additional file 4: Figure S3).
Similar(44)
When analyzing protein sequence alignments, one should make use of information on the alignment lengths and on the percent of each sequence that is involved in the alignment.
The links were weighted using the sum of the alignment lengths.
The program employed the following algorithm: the number of indel positions of pairwise alignments and the alignment length were computed.
Local conservation was avoided by dividing the alignment length of each aligned sequence by its total length.
The assignment of putative protein function was transferred if the alignment length of the highest ranking hit was over 50 amino acids and the identity over 25%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com