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As seen in Figure 3(a), the number of observed nodes N ( t ) displays a rapid increase in the beginning of the aggregation process, such that the aggregated network contains 90% of the nodes after t ∼ 30 days.
Although initiation of the aggregation process requires mHtt protein, aggregates can in turn recruit polypeptides with the wild-type polyQ stretch.
The Δ H of the aggregation process correlates with solubility, suggesting that the basic CPPs stabilize the aggregated state.
Because standard confocal smFRET techniques applied in solution have limitations in the detection of large species, we used single-molecule total internal reflection fluorescence (smTIRF) experiments to probe further the nature of the aggregates generated by fibril disaggregation and in the late stages of the aggregation process (see Extended Experimental Procedures and Figures S4C S4F).
The output of the aggregation process is one fuzzy set for each output variable.
Methods employed provided values for indicators at various stages of the aggregation process.
The focus lay on the first 2 h following initiation of the aggregation process which corresponds to the protofibril phase.
Controlled conditions and initiation of the aggregation process are prerequisites for the detection of transient intermediate states.
Besides intra-particle mesopores, over the course of the aggregation process an inter-particle (textural) large pore system is generated.
Characterization of the aggregation process should include intrinsic factors, such as sequence variation, and extrinsic factors, such as crowding effects.
The effect of polymer and salt concentrations on the kinetics and the thermodynamics of the aggregation process is considered.
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