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Second, the amount of starting material required was also prohibitively high in the initial stages.
The average RNA yield varied from 2.5 mg for dry seeds (0.05% of starting material) to 8 mg for germinating seeds (0.16% of starting material, stage Tmax).
Context: To ensure reproducible quality of herbal products, proper control of starting material is important.
Better control of lifetime can lead to higher degree of starting material utilization.
(C) Copy number profiles of mESC gDNA through TTAS, with different amount of starting material.
By changing the mixing ratio, we produced four types of starting material.
P21 and P22 were conducted with the same batch of starting material.
We first tested the lower limited of starting material for TTAS.
Equal loading of starting material was verified by monitoring the transcriptional activity of β-actin.
Samples with higher concentrations of starting material enter the logarithmic phase earlier than samples with a lower concentration of starting material and consequently, have a smaller crossing point value.
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The use of large gels, however, requires availability of larger amounts of starting material.
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