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This observation may indicate the existence of stage specific regulation mechanisms for different A-type variants.
The existence of stage specific, differentially expressed transcripts in a number of species is consistent with their regulatory role which is however mostly not elucidated.
Unexpectedly, we observed that, even prior to reprogramming, the HUF1 line possessed cells that demonstrated heterogeneous expression of stage specific embryonic antigen 3 (SSEA3; Figure 1B).
As such, this experimental design confounds the extrapolation of "stage specific" properties at baseline.
Thus, we used WL588 to assess the effects of stage specific β5 inhibition.
Mpss gene mapping and quantitative analysis of stage specific library sequencing was done using custom scripts written in PHP language.
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SMART-cDNA probes were evaluated for their temporal specificity using quantitative real-time PCR (qPCR) to amplify the transcripts of stage-specific genes (data not shown).
The osteoblast lineage is defined by the expression of stage-specific transcription factors.
Thus, several combinations of stage-specific transcription factors seem to be sufficient to differentiate hiPSCs into the OPC/OL lineage by directed overexpression.
This method allowed for isolation of stage-specific embryonic antigen-3-positive (SSEA-3+) and SSEA-3 cells from hESC cultures.
A new mouse model of stage-specific bone growth failure and fracture has been recovered as an autosomal recessive mutation, designated spontaneous fracture (sfx).
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