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Longer reads can provide more reliable information about the relative locations of specific base pairs.
As a result of specific base pairing, the RNAs present bind to the genes from which they were originally transcribed and produce fluorescent spots on the chip's surface.
Our results showing preferential association of MYCN to sites that are hypermethylated are not inconsistent with Perini et al. since our array results are not of sufficient resolution to ascertain the methylation status of specific base pairs.
Serological assay has a low cost and does not rely on PCR amplification, but it is still subjected to the effects of specific base mutation.
Analogous donors can be designed for the integration of in-frame protein tags or editing of specific base pairs with visible markers placed in adjacent introns.
Taking into account only homozygous mutations, our data also show that the presence of specific base changes is comparatively distributed among individual chromosomes and not abundantly located in a specific chromosome for both alleles.
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Since RNA-based gene regulation often involves the conditional formation of specific base-pairing patterns, RNA secondary structure prediction algorithms can be immediately converted into computational design tools for engineering RNA-based gene regulators.
"Due to host nation sensitivities and operational security, we are not detailing locations of specific bases of origin, aircraft or ordnance types," said Maj. Curtis J. Kellogg, a spokesman for Central Command, which oversees military operations in the Middle East.
The 400-mutation panel is dominated by insertions and SNVs (Fig. 3c, left) and is balanced in terms of specific base-pair changes (Fig. 3c, right).
This indicates a mutation proneness of specific bases at these two sites.
Identification of specific bases that are heterozygous in a given individual was accomplished using different methods for different sequencing technologies.
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