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Despite the natural rhizobia population has been large enough in the experimental soils, inoculation of selected isolated of native Bradyrhizobium significantly increased the pooled nodule number.
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Spirochetes isolated from ticks and wildlife are being characterized antigenically and genetically, and the infectivity of selected isolates for vertebrates and ticks evaluated.
The pipeline includes a rational sampling plan, isolation and cultivation of clonal strains, followed by a batch growth experiment designed to obtain information on robustness, growth characteristics, and the FA content of selected isolates during both nutrient replete exponential cultivation and nutrient limited stationary cultivation.
DNA sequencing of selected isolates was carried out to confirm morphological identification of P. kernoviae.
16S rRNA gene amplicons of selected isolates representative of each genotype were sequenced followed by phylogenetic analysis to find out their close relatives (Nandi et al. 2013).
Open image in new window Fig. 3 Maximum likelihood derived phylogenetic tree of selected isolates from contaminated soil and related organisms.
The level of diversity recorded in the bioethanol production capacity among the isolates means that a targeted screening of populations of selected isolates could greatly improve bioprocessing yields, in terms of providing both new host strains and candidate genes for the bioethanol industry.
Because river sediments were shown to be potentially important intermediate storage sites of fecal bacteria, that can be mobilized after re-suspension at increasing water discharge [13], special attention is given to this aspect by testing growth, survival, and mobilization of fecal bacteria after re-suspension of selected isolates.
Optical restriction mapping [31], [32], [33] was used to compare the overall genomic structure of selected isolates.
Toxinotyping of selected isolates was also performed (11 ).
The molecular types of selected isolates were identified by using previously described restriction fragment length polymorphism (RFLP) methods (6, 14 ).
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