Sentence examples for of scar sequence from inspiring English sources

Specific primers of SCAR (sequence characterized amplified regions) were then designed from these bands.

Based on the sequence information, a pair of SCAR (sequence characterized amplified region) primers was designed which generated a 600 bp amplicon (named OPF08-600) only in the apomictic plants.

The conversion of RAPD to SCAR (sequence characterized amplified region) markers facilitated MAS (e.g., indirect phenotypic selection, selection of hypostatic genes, gene pyramiding) for the development of bean germplasm or cultivars with enhanced resistance or combined resistance to several pathogens [ 6].

The latter strain, in which the hygromycin resistance cassette has been removed, has the C-terminal eleven residues of GroEL1, containing seven histidines, replaced by six non-histidine residues, which are part of the "scar" sequence left behind after removal of the resistance cassette.

Although there is limited homology of the scar sequence itself, it could provide a site for homologous recombination itself which might lead to problems and unwanted results in further manipulations.

After removal of tetRA, a "scar sequence" encoding the amino acid sequence MIPGIRRPAVRSSTSLGSIGTSKQLQPT plus the last six amino acids of the gene targeted for replacement is encoded in place of the original gene.

A smaller mutation that replaces approximately the middle third of ipaH9.8 with the scar sequence left behind after removal of tetRA is not polar.

The details for all E. coli constructs are described in Supporting Information Table 2. Genetic selection was performed using the library of ASKA clones where the GFP tag had been previously removed, leaving behind a C-terminal peptide scar sequence of GLCGR.

"frt" is the designation for the "scar" sequence remaining at the site of the cured Keio kan insertion.

However, the two modules are separated by a scar sequence, and the process is unsuitable for the assembly of multiple fragments in one step.

This observation suggests that the combination of the severe growth defect observed upon removal of the penultimate rrn operon and the presence of six identical scar sequences spread through the genome promote recombination events.