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Together, the pattern of rhythmic expression for these two bHLH-PAS transcripts was more similar to Drosophila, where DmClock exhibits peak expression in the light portion of a light:dark cycle, albeit at an earlier portion of this period (ZT = 0 4) [28], [28].
We found that although the ZT22 peak of Ucp1 mRNA was found in both control and jet-lagged mice, the ZT10 peak of Ucp1 mRNA was absent in jet-lagged mice, which correlated with a lack of rhythmic expression of Per2 and Bmal1 mRNAs in the same BAT (Fig. 4h i, Fig. S2d e).
Several other proteins, including kinases, play an important role in generation of rhythmic expression [ 7].
Coordinated non-random timing of peak and troughs of gene expression activity within a functionally related group can also serve as evidence of rhythmic expression.
Furthermore, the amplitude of rhythmic expression of MuRF1 [Trim63 (tripartite motif-containing 63)] and Fbxo32 genes, E3 ubiquitin ligases involved in muscle wasting, was increased [ 31].
These indications of a less robust rhythmic pattern are more pronounced at 12°C where several genes lose any trace of rhythmic expression pattern.
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The authors assessed the effects of this dissociation between sleep and biological time on rhythmic expression of the human peripheral transcriptome.
In other words, the PER/CRY complex inhibits its own expression, allowing for reactivation of BMAL1/CLOCK leading to rhythmic expression with a periodicity of 24 hours.
A recent study demonstrated that the temporal pattern of feeding, as well as the availability of food, greatly affected the phase of the rhythmic expression of the transcriptome in the liver, and even is capable of partially restoring rhythmic gene expression patterns in the otherwise arrhythmic Cry1−/− Cry2−/− mice [22].
The cyclic activation, repression, and reactivation of frq expression bring about circadian oscillation, which is the major basis of the rhythmic expression of CCGs.
The absence of nrl rhythmic expression in zebrafish larvae might also be explained by the fact that whole larvae RNA was used for qPCR analysis.
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