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Subsequently, participants were required to visit the clinic for collection of remaining samples.
The all rate of remaining samples was 98.88±1.031, with a maximum of 99.804 and a minimum of 94.277.
Samples with relapse events were randomly eliminated from the cohort until only 15% of remaining samples had relapse events (76/505 = 15%).
In addition, mutation analysis of remaining samples was abandoned, since this was deemed irrelevant as these harboured truncating APC mutations and are considered to be unlikely to also have CTNNB1 mutations [ 7].
RT-PCR single strand conformational polymorphism (SSCP) analysis of remaining samples failed to reveal any point mutations in the coding region of the gene, except for a polymorphism at codon 31 (Ser to Arg).
Similar(55)
Using our quality control (QC) rules, we determined a value for 11.2% of remaining sample measurements (n = 279), and only 5.9% of sample measurements (n = 147) were rerun.
Bayesian posterior probabilities (PP values) were calculated from the consensus of remaining sampled trees after excluding the first 10% of trees as burn-in.
Two-thirds of filtered water samples were free of faecal coliforms while most of the remaining samples had low levels of contamination.
Three of the samples (two control pigs and one diseased pig) were clearly positive whereas a number of the remaining samples had faint bands of the correct size.
Instead, for whatever reason, as the Free Press noted, a quarter of the remaining samples "came from a single stretch of Flushing Road in Flint," which had a water-main section only a few years old.
Of the remaining samples, 254 were available for studies of human caliciviruses, and 30 were positive.
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CEO of Professional Science Editing for Scientists @ prosciediting.com