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Meanwhile, the Turkish government has signed a landmark set of preliminary protocols with Armenia.
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We considered clinical trials that met our pre-determined list of inclusion criteria that were reviewed and approved as part of the preliminary protocol.
Murphy, D. J., Hall, C. A. S., Dale, M. & Cleveland, C. Order from chaos: a preliminary protocol for determining the EROI of fuels.
A preliminary protocol was designed and independently reviewed.
A preliminary protocol was designed and then independently peer-reviewed.
PCR reactions were performed using the following protocol: 3 min of preliminary activation of Taq polymerase at 95°C were followed by 32 (34 for nuclear genes) three-step cycles of denaturation at 94°C (30 sec), annealing temperature specific for primer (30 sec; see Additional file 1: Table S1) and extension at 72°C (70 sec), and a final extension at 72°C (10 min).
The use of membrane integrity dyes is a well-accepted method of preliminary assessment of cell recovery after experimental protocols.
The protocol then began with three sessions of preliminary testing in order to determine certain key variables.
A couple of preliminary questions suggest themselves.
Development of preliminary draft.
The resultant reaction buffers of preliminary real-time PCR were directly put in TA cloning using pTAC-1 vector (BioDynamics Laboratory, Tokyo, Japan) according to the manufacture's protocol.
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