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Of each S. enterica serovar 50 μl of 107 CFU ml−1 of phosphate buffer was added to 0.5 ml tubes.
This step was followed by two quick washes with 80% ethanol and then incubation with high concentration of phosphate buffer at 4 °C for 30 min.
Cells were collected on nitrocellulose filters (Millipore, 0.8 µm pore size; EMD Millipore Corp., Billerica, MA, USA), and 100 µL of phosphate buffer was dropped on the filter.
All mice received Rg1 or an equivalent volume of phosphate buffer saline (PBS) intraperitoneally 1 h before LPS administration.
Ionic strength of phosphate buffer.
The chemicals used for preparation of phosphate buffer and solutions were of analytical grade.
The effect of changing the pH of phosphate buffer solution was tested from 3.0 to 7.0.
Stability assessments were performed at different pH of phosphate buffer varying from pH 3 to 7.4.
Decreasing or increasing the ionic strength of phosphate buffer results in lower resolution or overlapping peaks.
The dialysis bag was placed in 50 mL of phosphate buffer of pH 7.4.
The pellet obtained was resuspended in 1 mL of phosphate buffer (pH 7).
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