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We showed that heat treatment decreased infectious MERS-CoV below the detection limit of our titration assay, and this might function as a relatively easy and cost-effective measure to prevent transmission.
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When the two effects were combined, only weak binding could be observed over the range of our titrations, indicating a Kd value greater than 4 μM.
Our titration of crude cell extracts of cells in which expression levels of N-S-β were induced with 0.01, 0.02, or 0.05 mM ara revealed 1 Y per β and 2 Fe atoms per β!
Strikingly, however, viral budding was extremely rare in case of rA75/17red-infected rA75/17red-infectedur titration assays in supernatants.
Our titration of the suspension used by Tigertt et al. is based on the assumption that the two strains are equally infectious in guinea pigs.
The analytical sensitivity (titration) of our test was comparable to the diagnostic sensitivity (clinical samples) better than, the real-time PCR or nested PCR.
Independent synthesis and titration of our original HTS hits showed that the two epimeric alcohols 1 and 28 had similar potency (GI50 = 120 and 62 nM, respectively), while desmethyl ethanol derivative 30 lost some activity (GI50 = 630 nM).
In order to test the sensitivity of our approach, we performed a titration experiment.
Most of our initial concerns about such titrations dealt with the electrode, whether it would function properly, be stable, and give meaningful values.
Our primary titration target for determining best PEEP was CLUNG, but its behavior paralleled the responses of a variety of titration targets, both at normal IAP and during IAH.
Therefore standardization of the exact procedure of titration remains of uttermost importance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com