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To characterize the SERS performance of our substrates, benzene thiol was used as the probe molecule.
Finally, the application of our substrates as SERS bio-sensors was demonstrated with the detection of the neurotransmitters acetylcholine, dopamine, epinephrine and choline, the precursor for acetylcholine.
To characterize the SERS performance of our substrates, commercial Klarite® substrates were used as reference samples which consists of gold-coated textured silicon (regular arrays of inverted pyramids of 1.5-μm wide and 0.7-μm deep) mounted on a glass microscope slide.
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The large surface area of our substrate is induced by the high density and large depth of the nanogap structure.
Overall, the specific characteristic of our substrate peptide library makes it a rapid tool to high-throughput screen for novel substrates to detect bacterial proteolytic activity.
As shown in Figure 3a, an obvious background signal is found in the Raman spectrum of the Klarite® substrate, which almost cannot be found in the Raman spectrum of our substrate.
In other words, the high density and large depth of the nanogap structure of our substrate provide dense strong 'hot spots' and an enormous Raman intensity but yields a relative small average EF.
The results of our substrate engineering technique, which involves implantation of nitrogen into Si through an AlN thin film, have shown to simultaneously and significantly reduce the dislocation density and macro-cracks in epitaxially grown 2 μm GaN films.
The intensive SERS spectra observed for low concentrations of choline (2 × 10−6 M), acetylcholine (4 × 10−6 M), dopamine (1 × 10−7 M) and epinephrine (7 × 10−4 M) demonstrated the high sensitivity of our substrate.
Having demonstrated the selectivity of our substrate, we next wanted to determine if it could be used in a low-volume, 384-well plate assay.
This positioning of serine, as well as the coincidental fact that the amino terminal residue of our substrate is glycine, is strongly reminiscent of the consensus sequence for N-terminal glycine myristoylation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com