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To determine the purity of our sorted cells, we isolated both ALDHhigh and ALDHlow cells (Figure 2).
Side scatter analysis (figure 2B) of our sorted population revealed larger cells express higher levels of CD31, indicating its expression is relative to cell size.
Knowing that we might miss genes that are expressed in subsets of our sorted neuronal subpopulations, we assumed that, below 1,000 number of reads, transcripts' detection by ISH would be difficult to monitor.
We identified LDHB, one of the two human lactate dehydrogenase (LDH) subunits, at the top (position 1 of 42,069 entries) of our sorted list of combined RTP+ and PTS1 scores (see Schueren et al., 2014 for Dataset 1).
The moderate sizes of Pareto fronts in our applications also imply that no benefit is to be expected from using quad-tree data structures in place of our sorted list representation.
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Not all of the 251 species on our sorted list were represented in the NHM collection, although the majority of genera (∼90%) were.
We were unable to test the presence of CACs in our sorted CD133+ fraction due to a lack of specific "human" CAC marker.
We used MISFITS (Nguyen et al. 2011) to determine the occurrence of site patterns in our sorted alignment that were unexpected under a general time reversible (GTR) + G model using three alternative Gnetales phylogenetic trees incorporated as part of the evolutionary model.
Although a few reports describe co-assembled OPV and PBI mixtures17, 43, 44, to the best of our knowledge, self-sorted aggregates in water have not been reported.
With the Higgs boson pretty much sorted, many of our brightest minds will be turning to another experiment into an enduring scientific mystery, this time occurring deep within the most unknowable parts of the BBC.
It has been reported that IGF2 transcription is induced by prolactin [ 38, 42], and our analysis of sorted cellular subsets from WT mammary glands demonstrated that IGF2 is produced specifically in hormone-sensing cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com