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Accelerometry is not only being used in healthy community dwelling older adults, as in the current study, but also in a variety of older specific populations (e.g., adults, older adults after stroke, or in hospital), and appropriate assumptions for these groups may need to be condition-specific.
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If any single theme unites these otherwise disparate voices, it is the retelling and reintegration of older, culturally specific mythologies – from African folktales to Chinese legend – into to the science fiction canon.
This may have accelerated the obsolescence of older workers' specific knowledge about how to perform this kind of activities before the introduction of these new tools.
0.4 mL of the suspension was inoculated into the yolk sac of 7-day-old specific pathogen free embryonated chicken eggs.
One virus strain, designated YY5, was isolated from the Shaoxing duck after inoculation of clarified theca folliculus suspension into the allantoic cavities of 9-day-old specific pathogen free chicken embryos.
The virus was propagated by inoculation in the allantoic cavity of 8-10 days old specific pathogen free (SPF) embryos (Lohmann Tierzucht, Cuxhaven, Germany).
Samples that showed a positive reaction in the partial M segment RT-PCR were grown in the allantoic cavities of 10-day-old specific pathogen free embryonated chicken eggs.
We attempted to isolate the virus by injecting the chorioallantoic membranes (CAM) and allantoic cavities of 9-day-old specific pathogen free (SPF) chicken embryos with the scab specimens that were positive for FWPV.
Samples positive for H7N9 virus by rRT-PCR were inoculated into the allantoic sac of 10-day-old specific pathogen free embryonated chicken eggs and incubated for 48 72 h at 35°C for virus isolation (13 ).
To evaluate pathogenicity of W24 and 6D18 in chickens, we inoculated groups of ten 6-week-old specific pathogen free chickens intravenously with a 10 median egg infective dose of each virus in a 0.2-mL volume of phosphate-buffered saline; deaths were observed over a 10-day period (7 ).
Virus pathogenicity was evaluated by inoculation of six 4-week-old specific pathogen free chickens with 6 log10 50% egg infectious doses of A/duck/Laos/XBY004/2014(H5N6) from egg allantoic fluid by the oral nasal ocular route.
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