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For a series of nuclei, values were averaged to produce curves as shown.
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The apoptotic cell number reported is the mean of the two apoptotic nuclei values per tumor.
Similarly, aneuploidy has been diagnosed when there are additional well-defined aneuploid peaks or when the number of nuclei with DI values in the aneuploid region constitute >1% of the total cells [ 4].
Surface renderings of the p.d.f for phospho-MEK signal intensity within the cell (e) cytoplasm and (f) nucleus of segmented cells, plotted perpendicular to the normalized distance values of nuclei centroids (note the plots in surface renderings of the p.d.f are analogous to the 'whole data clouds segmented by spatial position', as shown in Additional file 2 : Figure S3e and S3g in pMEK.png).
Recently, it had been demonstrated that in follicular adenomas the chromatin texture was size dependent, when analyzing digitalized, gray-value transformed images of nuclei in routinely hematoxylin-eosin (HE -stained paraffin sections.
The mammalian amygdala is a heterogeneous collection of nuclei involved in the formation of stimulus-value associations and the storage of the emotional aspects of memories.
Images of metastatic nodes have fewer low pixel values due to crowding and enlargement of nuclei and therefore have lower skewness.
Using the value obtained, an estimate of the amount of DNA in the 1 ml of nuclei was calculated by multiplying a by 20 for the volume of nuclei sample initially used for quantification.
The mean amount of nuclei counted was 683 in concordant tumours and 308 in discrepant tumours for average values (P<0.0005).
The cut‐off values were calculated as the mean percentage ± three standard deviations of nuclei showing loss of both 9p21 signals for homozygous deletion, as previously described.
The software used to quantify the DNA content made possible to verify the percentage of nuclei with DNA content higher than 5C, and to observe the minimum and maximum values of DNA content in all classes analyzed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com