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The volume was then estimated by assuming an average 5-to-1 ratio of myocyte width to thickness (volume (μm) = myocyte area/myocyte length/5) and converted to pl.
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Quantitative measurements of myocyte width yielded identical values in all groups (data not shown), which confirmed the absence of hypertrophy at this stage.
Morphometric analysis showed a significant increase in average myocyte width of groups treated with succinate compared to controls, (12 ± 0.10 μm in control vs 14.7 ± 0.10 μm in succinate treated animals, p < 0.001),.
To examine myocyte width, wheat germ agglutinin (Texas Red-X conjugate; Molecular Probes, Invitrogen) was used to stain cell borders.
This means that the ratio of waist width to hip width substantially underestimates the true WHR.
This was associated with increased myocyte width, in particular thickening of the left ventricular free wall and accompanied by increased expression of molecular markers of myocyte hypertrophy.
The myocyte width was also significantly higher in the Mat-Ob group (Fig. 2B; P < 0.0001).
Frozen tissue was sectioned for hematoxylin and eosin staining and myocyte width analysis.
Impairment of myocyte ATPases leads to fatigue [ 4].
BACKSCAL is proportional to that width, or to the ratio of the background region width to the source-region width.
Stretching of myocytes leads to release of angiotensin, which is known to induce a hypertrophic response in the myocyte through autocrine/paracrine effects.
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