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The production of multiple signalling peptides from one precursor is a common feature found in animals.
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Another example of the multiple functions of signal peptides is the fate of signal peptide fragments of HIV-1 gp160 and of the human preprolactin, which interfere with cytosolic calmodulin, a major protein involved in the regulation of calcium metabolism [17], [19], [20].
Indeed, the evolution of metazoans coincided with an expansion of GPCR families from the complement found in their nonmetazoan ancestor to those that would bind multiple types of signaling peptides such as the CKH-like family.
Consequently, the identification of multiple peptides is not due to shared fragments between peptides.
Therefore, probing into multiple signalling pathways will be necessary to determine the pathway that G7-18NATE peptise invadinginvading
The amino acid sequences of these three signal peptides show a high level of similarity using the PRALINE multiple sequence alignment tool (Simossis et al. 2005).
With regard to Set I novel signal peptides, dsbAss and pelBss were selected to initiate the design of new signal peptides.
Interfering signals from unrelated peptide species compromise MRM-MS assays, requiring the monitoring of multiple peptide precursor-fragment transitions to increase specificity when quantifying a particular peptide analyte.
The data showed that some of the modified signal peptides improved the secretion efficiency of ATH35L compared with E. coli signal peptides from dsbA, pelB and phoA proteins.
Multiple alignments of protein and DNA sequences were performed using ClustalW (http://www.ebi.ac.uk/Tools/msa/clustalw2/). The prediction of signal peptides in protein sequences was carried out using the SignalP 4.1 Server (www.cbs.dtu.dk/services/SignalP/).dtu.dk/services/SignalP/
The RPSP is a fast web service for detection of signal peptides in proteins.
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