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Seven parameters temperature, pH, percentage of medium volume per flask volume (% v/v) and glucose, KNO3, K2HPO4 and soy isolate concentrations affected the biomass and ARA production of this fungus and were determined using the Plackett-Burman statistical design technique.
Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
Previous studies showed oxygen concentration was declined with the increase of medium volume in shake flask due to change of oxygen transfer area (Dou et al. 2013).
Bretons have a well-proportioned head of medium volume with a straight profile and a strong, short neck well-set into muscular withers.
Thus, before starting experiments, we had examined the effects of medium volume, of different media, and prolonged culture in a flask with continuous exposure to spermatozoa in terms of embryo development.
One-third of medium volume was exchanged every 5th day.
Similar(52)
The proportion of patients treated in low-volume hospitals dropped from 22%to12%2%, with a concurrent increase in the activity of medium-volume providers.
The ratio of cell number to medium volume was 1.7 × 10 cells/3 mL in all experiments (dynamic and static conditions).
It was added to each well of 96 wells plate, a volume of 15 μl Alamar Blue solution, which represent 10% of the medium volume in each well.
Using the phase mask allows effective use of the medium volume and its dynamic range and improves reconstruction performances of stored holograms.
The EBCT and organic loading rates were recorded on the basis of the medium volume.
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