Outside of Australia, there are only a handful of known quantities.
This contributes to the impression that Ms. Thater's latest effort does little more than juggle a series of known quantities and conventions, from reliably sensational subject matter to technologically savvy video environment.
Protein spot densities of representative slides were measured by using an anti-GST antibody and compared to a dilution series of known quantities of protein also printed on each slide.
To quantify total TH, in house standards (calibrated PC12 cell extract) are used to interpolate sample immunoreactivity to anti-TH versus a standard curve of known quantities of TH and the value is expressed as ng TH per ug total protein loaded [25], [27], [50], [51], [57].
Sensitivity of the QPCR assays: To estimate the sensitivity of the QPCR procedure, a plasmid DNA control which contains part of the H19 cDNA region was used with 10-fold serial dilutions of known quantities from 0.2ng (9×107 copies) to 0.2×10−7 ng (≤9 copies of plasmid DNA) for H19 analysis.
Hydrolysis of known quantities of free peptides was used as standard.
Additionally, control libraries of known quantities were prepared in various concentrations for standard curve generation.
Standard curves, using plasmid dilutions of known quantities as templates, were generated for each gene in each qPCR experiment.
The final amount of gDNA in the sample was determined by interpolation from a standard curve of known quantities of commercially available gDNA (Becton Dickinson, Franklin Lakes, NJ, USA).
Spiking of known quantities of the corresponding bacterial mRNAs into the labelling mix allows to estimate the linear range and sensitivity of our arrays.
