Fumaric acid and carbohydrate standard solutions of known concentrations were used for calibration.
H2O2 content was computed from a standard curve prepared from H2O2 solutions of known concentrations.
Glucose solutions of known concentrations (100, 200, 400 and 600 μg/mL) were used as standards.
Calibration plots were established for a series of stock solutions of known concentrations of proteins.
The amount of n-hexane produced was quantified by a standard curve of known concentrations of n-hexane.
The concentration of each target was determined by interpolating the Ct value from respective standard curves of known concentrations.
Comparison of known concentrations of individual targets in mixtures to those estimated by the solution revealed highly consistent results.
The glucosamine value was calculated back from the absorbance measurement using a standard curve of known concentrations of N-acetylglucosamine (Sigma-Aldrich, Dorset, UK).
Before testing the unknown concentration, the standard curve was plotted with standard solutions of known concentrations, i.e., 0.0, 0.5, 0.5, 1.0, 1.5, and 2.0 ppm.
At any fixed λ max, the absorbance values of various solutions of known concentrations (reference sample) were calculated by the machine.
H2S was calculated based on a standard curve of known concentrations of NaHS and expressed as nmol g-1 fresh weight (FW).
