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But, because our target was to be able to predict the function of the gene from the sequence of its transcript, it was necessary to select protein-coding transcripts efficiently.
An actin gene (protein_id 139298) was used as endogenous reference gene, which was not valuable in quantity of its transcript among the culture conditions used in this study (Figure 1).
Alternatively, failure to detect LMP1 protein could be due to aberrant mRNA splicing of its transcript (see below).
To begin the analysis of cold function, we studied by in situ hybridisation the embryonic distribution of its transcript.
Confirming the identification of two of its transcript variants in the microarray, RCAN1 mRNA content was highly elevated at 3H (16.6-fold, P<0.001) (Fig. 1A).
Hence, we hypothesized that reduced amounts of C/EBPα protein might determine a poorer stimulation of the hepcidin promoter, therefore accounting for the down-modulation of its transcript levels at this time-point.
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The simulator randomly chooses a (user-specified) number of genes, and for each gene, it samples a subset of its transcripts.
The transcription of both suffix strands and the degradation of its transcripts by RNA silencing mechanisms are developmentally regulated.
NPR1 is constitutively expressed and levels of its transcripts increased only two-fold following SA treatment, suggesting that it is regulated at the protein level [ 7].
The expression pattern of DREB1A evidenced a consistent increment of its transcripts at dark (ZT20) (Figure 2, cluster 5), possibly due to the decrease of ambient temperature at the presumptive dusk (ZT16) (see Material and Methods).
Gene Ontology analysis using BLAST2GO revealed P. carinii dedicated most of its transcripts to cellular and physiological processes (∼80%), molecular binding and catalytic activities (∼70%), and were primarily derived from cell and organellar compartments (∼80%).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com