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The inclusion of additional sequence data from the secA and hsp65 genes resulted in the assignation of 26 M. abscessus isolates, 7 M. massiliense isolates, and 2 M. bolletii isolates; the remaining 7 isolates ultimately were assigned to M. massiliense via use of ITS sequencing.
The first human genome, published in 2003, took more than a decade to complete, but the 1,000 Genomes Project completed the bulk of its sequencing work in less than a year.
Eric Lander, professor of biology and director of the Whitehead Genome Center, and Lauren Linton, co-director of its sequencing center, as well as sequencing center team leaders were in the White House East Room as President Clinton and Britain's Prime Minister Tony Blair made the historic announcement -- that the "book of life" had been decoded.
One of the 125 parent genotypes was eliminated from genomic selection analyses, owing to poor quality (as number of reads) of its sequencing data.
For each detected SNP, we checked all reads in each individual mosquito regardless of its sequencing quality score (Phred score) [ 36].
We selected Newbler for Ion PGM because it is known to produce longer contigs for Ion PGM as well [ 22] because of the similarity of its sequencing chemistry to that of Roche 454.
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The incorporation of ITS sequences of human pathogenic Candida species into public nucleotide sequence databases and the reliability of ITS sequence data were also addressed.
As GenBank holds mainly ITS sequences for this genus, we firstly compiled a dataset of ITS sequences to investigate the species diversity of Hydnum.
Since then, over ten phylogenetic species within the species complexes of H. rufescens and H. repandum have been identified by analyzing the divergences of ITS sequences of European samples4.
Phylogenetic analysis of ITS sequence among Phellinus species showed the isolate of P. noxius were in the same clade distinctly separated from other Phellinus species.
Phylogenetic analysis of ITS sequences among Phellinus species showed that isolates of P. noxius were in the same clade distinctly separated from other Phellinus species (Fig. 2).
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