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The genome appears to be extreme in many different aspects: It has lost over 40% of its annotated coding capacity, massively rearranged its genome, become extremely AT rich and acquired ten novel ORFs that constitute over a quarter of the total mtDNA.
The ecology of this pathogen outside its human host is largely unknown and one third of its annotated genes are still hypothetical.
One possible example is the Tex16 gene whose putative un-annotated TSS lies ~200 kb upstream of its annotated 5′ end (Additional file 1: Figure S11).
Importantly, this list contains the β-globin gene, as three unique CLIP reads were mapped in the proximity of its annotated poly(A) site, in the transfected sample.
A popular strategy has been to cluster the network into functionally coherent groups of proteins and assign the entire cluster with a function based on functions of its annotated members.
Short-read mapping is a basic step in RNA-Seq data analyses, and to a certain extent, the percent of reads mapped to a given transcriptome can roughly reflect the completeness of its annotated genes and transcripts.
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Moreover, an alignment is called a pure alignment if it satisfies two conditions: (i) it contains at least three MIPS annotated proteins and (ii) there exists a complex in MIPS that covers >75% of its MIPS annotated proteins.
With 79% of all A. nidulans genes being organized in Jaccard orthologous clusters covering 97% of all its GO annotated genes, we demonstrated that this approach is promising for mapping GO annotation between closely related genomes such as those of the genus Aspergillus.
But we cannot expect complete absence of transcription outside of annotated genes either.
In the S. erythraea genome, more than 45% of its ORFs are annotated as hypothetical proteins [ 17].
pCS2 is clearly phage-derived, with 20 of its 52 ORFs annotated as phage genes, including multiple genes encoding structural phage components.
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CEO of Professional Science Editing for Scientists @ prosciediting.com