Haploid plants of DMSW were successfully obtained in this study induced by both maize pollens of inducer line and normal inbred line.
Upon characterization, the cells were induced to differentiate into smooth muscle (SM -like cellSM -likea variety of inducellsombinations.
KCN was added to various concentrations of inducer.
(b) Post-induction temperature and post-induction period at fixed level of inducer concentration.
In the absence of inducer, promoter activity is fully repressed; addition of inducer rapidly increases gene expression by four to five orders of magnitude.
The optimal induction conditions to be determined consisted of inducer concentration and dry cell weight at the time of induction.
This allowed for protein production at high biomass levels without the addition of inducer.
Each line color represents one concentration of inducer and is a subtraction of the control from the treatment.
From the RSM, the concentration of inducer, cultivation time, and culture temperatures had significant effects on the MFS production, with the effect of inducer concentration being more pronounced that other factors.
Since protein expression from these systems is triggered by exogenous inducer, periodic intravenous injection of inducer is necessary.
The region of inducer concentrations where bimodality is observed is the same and independent of GFP half-life.
