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We have found it necessary to take measures of increasing stringency, not only against enemy aliens and suspicious characters of other nationalities, but also against British subjects who may become a danger or a nuisance should the war be transported to the United Kingdom.
These are, in order of increasing stringency, intelligibility, accessibility, and shareability.
Proteins of different solubility were extracted from brain in buffers of increasing stringency, using a slightly modified protocol previously described [24], [25].
Replica plating was performed under conditions of increasing stringency according to the manufacturer's suggestions and subsequently analysed for growth on –Leu/-Trp/-His, -Leu/-Trp/-His/-Ade synthetic drop out (SD) media.
However, the rules above, such as they are, are given in order of increasing stringency: in general search queries of type 1 will return more results than those with 3. The more common an individual's name, the more stringent the rule used must be this is our general observation and recommendation, but does not guarantee improved results.
After a succession of one hour washes (2xSSC/1 mM DTT, 50°C; 0.2xSSC/1 mMDTT, 55°C; 0.2xSSC/1 mM DTT, 60°C), the tissue was treated with RNase-A and washed under conditions of increasing stringency, including a 30 min wash at 60°C in 0.1xSSC.
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Selection of phages with specificity for nmAb-KT was based on increasing stringency of selection at each round of panning.
IKJ5: The data on increasing stringency of the motif from 8to1010 bp is reported as being shown in Fig 1.
Irrespective of the introduction of increased stringency measures, the abundance of STC1 and LUM was significantly higher in both in vivo and in vitro matured oocytes compared to 0 h (GV stage oocytes).
Notwithstanding, the constructed library was screened using a traditional protocol consisting of successive rounds of panning, with increasing stringency due to an increasing number of washing steps between rounds.
Matings for all clones yielded diploids that formed colonies with a consistent pattern, with the number of colonies decreasing with increasing stringency of the selection medium (QDO being the most stringent, and leucine-deficient SD medium being the least stringent).
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CEO of Professional Science Editing for Scientists @ prosciediting.com