Sentence examples for of highest stringency from inspiring English sources

Expectedly we find that the number of predicted EREs increases with the stringency of the observed sites, with 50% of highest stringency sites showing a good match to a full ERE.

Each of the three regions was detected in all VLM-producing strains under conditions of high stringency (Fig. 1c).

The screening was successfully re-performed with a single round of high stringency.

The importance of high stringency assay development in this sector cannot be overestimated.

The use of high stringency PCR cycles encourages the efficient and specific amplification of the target products, while off-target PCR products are progressively diluted.

Use of high stringency parameters resulted in an average 96.33% concordance rate of SNP calls between the two DNA sources (Table 2).

The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency.

Probes were hybridized overnight under conditions of high stringency at 65°C and detected by using chemiluminescence as recommended by the manufacturer.

However, a real BAC contig could be split into two contigs or more when we use assembly parameters of high stringency, especially for those genome regions with higher levels of heterozygosity.

Plasmids that hybridized with a biotinylated probe were bound to streptavidin coated magnetic beads (Dynabeads® M-280 Streptavidin, Invitrogen) and then subjected to a series of high stringency washes to remove any plasmids that non-specifically hybridized with a probe.

Reactions were carried out in a thermal cycler (Perkin-Elmer) with five cycles of low stringency (94°C for 30 s, 40°C for 60 s, 72°C for 1.5 min), followed by 30 cycles of high stringency (94°C for 15 s, 55°C for 15 s, 72°C for 1 min).