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Second, as large females usually produce larger offspring of higher viability (Trippel 1995; Walsh et al. 2006), a decrease in growth could impair the recruitment and consequently the long-term yield of the population.
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In the present study, we report on the combined efforts of material chemistry, engineering and biology as a systemic approach for the fabrication of high viability 3D printed macroporous gelatin methacrylamide constructs.
This supports a similar finding of high viability of male unreduced gametes in Arabidopsis [ 27].
The use of a purely mechanical procedure was the most time and cost effective, providing cell pools of high viability.
Both strains showed comparable levels of high viability, development to adulthood, and fertility for 60 days (Fig. 5).
These fragments formed at cell cell junctions, but gentle dispersal of the cells, to form a single-cell suspension of high viability, caused the release of most of the fragments.
However, a tendency of a higher viability of HBECs was observed when they were cultured in triplicate (Fig. 4A, one-way ANOVA, p = 0.07, n = 3).
This could be attributed to the maintenance of a higher viability and better physiological state of the cells, as explained earlier, by providing fresh medium and removal of potential inhibitors during perfusion.
Improved maintenance of endothelial function and higher viability of saphenous vein grafts stored in a complex tissue culture medium (TCM) have been demonstrated.
The results of MSCs viability indicated a slightly higher viability of HAP coatings (72% vs. control) compared to HAP-PU composites (69% vs. control) synthesized at high pressure (60 atm).
The optimal agitation rate for the culture of Ecad-/ ES cells was considered to be 140 rpm due to high viability of the culture over 72 h.
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