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The yield and purity of the biotinylated aRNA samples were determined in a BioPhotometer (Eppendorf) and only samples of good integrity were further processed.
Higher FA and lower RD or MD are commonly used indicators of good integrity of fiber bundles [ 44].
Only samples of good integrity were further used and 15 μg of the fragmented samples were hybridized to GeneChip® Rat Genome 230 2.0 Arrays (RAT230_2 chip, Affymetrix) and subsequently scanned.
Large RNA integrity was assessed using the RNA 6000 Nano LabChip Series II Assay with the 2100 Bioanalyzer System (Agilent Technologies, Palo Alto, CA, USA) which generated the RNA integrity number (RIN) to ensure that only RNA of good integrity was used in these experiments (RIN range 7.0 - 9.5).
Electropherograms and gel-like images were evaluated using the Agilent 2100 Expert software (version B.02.03; Agilent Technologies, Palo Alto, CA, USA), which generated the RNA integrity number to ensure that only RNA of good integrity was used in these experiments (RNA integrity number range, 7.6 to 9.5).
KTP and its isomorphs pole anisotropically, favouring domain growth along [010] on the conventional setting of the cell with a = 12.819 (3), b = 6.399 (1), c = 10.584 (2) Å (Thomas et al., 1990 ▶). Gratings of good integrity were produced with walls designed to be parallel to (100) planes (a-poled), whereas the b-poled gratings with walls parallel to (010) were of a poor quality.
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According to the study by Condé-Green et al, 13 the aspirate processed by washing not only maintained considerable number of adipocyte with good integrity deprived of blood cell contaminants but also preserved a great number of endothelial and mesenchymal stem cells, which are believed to contribute to increased neoangiogenesis, reduced apoptosis, and promotion of adipocyte differentiation.
Integrity of total RNA samples was assessed by denaturing formaldehyde gel electrophoresis, where the presence of sharp 28S and 18S ribosomal RNA bands at an intensity ratio of ~2:1 (28S:18S) indicated good integrity.
All of the RNA samples from spent medium had a RIN value greater than 7.5, indicating good integrity of the rRNA.
Higher FA and lower RD or MD represents good integrity of white matter [ 44], which have been found in male CD individuals relative to healthy male controls [ 18, 19], indicating an atypical maturation of CD individuals' white matter.
Developing composite films with good integrity is of great importance for the requirement of effective protection of metal materials.
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