(B) Time-course quantification of Gag vacuole formation by ΔNC-Gag as compared with wildtype Gag in MiR+ cells.
Expressing the results as a percentage means that the proportion of GAG released was quantified in relation to the total amount of GAG in each explant.
The reduction in virus release, despite the similar total level of Gag expression in MiR+ and Ctrl cells, raised the possibility that non-silencing miRNAs function to alter intracellular distribution of Gag complexes.
Heterogeneity of Gag mutational pathways in primary HIV-1 subtype C infection.
Non-infectious particles formed by the expression of Gag alone, or newly budded particles before maturation.
The NC domain of Gag is the primary viral determinant that drives RNA packaging.
The current size of different domains and the number of GAG chains are not respected.
To allow sufficient modification of GAG chains, a G3 domain is essential.
That's the kind of gag you would expect to be pulled by Vegemite.
Applicants really have no choice but to attempt some sort of gag here, though.
The release of WT CCL5 was also reduced after inhibition of GAG presence/synthesis by intracellular expression of heparanase, inhibition of GAG sulfation, and sulfate deprivation.
