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The expression of functional members of the TaHKT2 1 gene family were investigated by imposing NaCl treatments on Chinese Spring seedlings and detection of transcripts specific for each gene in roots, sheaths and leaf blades.
Only a small pseudogene fragment of the C. biroi ortholog remains (CbirObpfrag4), and, due to the lack of functional members of this clade in C. biroi, we could not determine antenna specificity.
Table 3 summarizes the number and type of cis-acting regulatory elements (CREs) identified in promoters of functional members of the TaHKT2 1 family using the PLANTcare and PLACE databases.
at 72°C.> -wrap-foot> All of these primers were designed as part of this study based on sequences of functional members of the TRAV/TRDV subgroups identified in the UMD3.1 genome assembly, with the exception of DV3 which was published previously [ 17].
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They are best recognized by comparison to functional members of the family which allows the detection of frameshifts and premature truncation.
Ultimately, this has led to the idea of 'functional metagenomics', defined as 'the characterization of key functional members of the microbiome that most influence host metabolism and hence health' [ 52].
However, CGI-58 is unlikely to exhibit hydrolase activity due to the fact that asparagine-153 (N153) in CGI-58 replaces a nucleophilic serine residue that is required in the active site of enzymatically functional members of the esterase/thioesterase/lipase family.
In the present work, quantitative real-time polymerase chain reaction (RT-PCR) was used to detect transcripts of all of the nine functional members of the UGT1A locus in 23 normal human tissues.
The proportion of putatively functional members in the V2R sequences in zebrafish (82%) and fugu (78%) were higher than in medaka (56%) and pufferfish (52%).
Through merely talking about these things, or expressing ourselves creatively, we create healthy outlets and increase the numbers of successful and functional members of our communities.
However, VH5 51, one of only two functional members of the VH5 family, is also found in large numbers here.
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