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Characters assumed to be at least to be of functional interest are thrown overboard.
Nine genes of functional interest were selected for validation by quantitative PCR (qPCR) analysis (Table 2).
Furthermore, we selected nine candidate genes of functional interest upregulated in VMN samples for validation by sequencing and qPCR (see below).
The nearest gene other than IRGM of functional interest at this locus is TNIP1, which is located 80 kb distal to the region of association.
Pathway analyses identified several GO categories of functional interest, six of which survived multiple testing (GO 0004112, GO 0004114, GO 0042166, GO 0042611, GO 0008537 and GO 0042405; Table 4).
Activation of CFTR in endothelial cells could be of functional interest in transendothelial transport, in pH regulation because of the CFTR permeability for bicarbonate, and intriguingly also as part of the NO signaling cascade because of their sensitivity to cGMP, which might be elevated during endothelial NO production.
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The Bonferoni Post Test for selected pairs of data groups was used to test two pairs of data groups of special functional interest (Prism 3.0).
Furthermore, a significant 20- to 100-fold increase in CYP450 class-1, a nearly 20-fold upregulation of SERPINB5, and a 60% downregulation of BIRC5 gene expression are of special functional interest.
This excludes the isolated insertion sequences of the RGPs, but favors regions with several genes of potential functional interest in the bacterial biology.
Of potential functional interest, we noted that CRA was associated with significantly higher abundance of fecal Proteobacteria, the phylum that includes established gut pathogens — Pseudomonas, Escherichia, Shigella, Salmonella, Serratia, Klebsiella, and Helicobacter.
SERPINB5 and CYP1A1/1A2/1B1 are transcripts of special functional interest.
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