Sentence examples for of fixed zebrafish from inspiring English sources
Immunostaining of fixed zebrafish embryos was performed as described (21).
Images of fixed zebrafish brains were acquired using a Leica fluorescence dissection microscope.
For imaging of fixed zebrafish, the embryos and larvae were mounted in 100% glycerol.
By immunizing with the ectodomains of zebrafish cell surface receptor proteins expressed in mammalian cells and screening for formalin-resistant epitopes, we selected antibodies that gave expected staining patterns on wholemount fixed zebrafish embryos.
fixed = number of fixed AFLP-fragments; Frag.
Fortunately, however, we have recently observed that at 3 days post-fertilisation, zebrafish embryos are sufficiently transparent for the direct application of (FLIM) OPT. Figure 8 e shows a 3D fluorescence lifetime image of a fixed but uncleared transgenic (FLI GFP) zebrafish in which the vasculature is genetically labelled with EGFP.
The properties of HCR lead to straightforward multiplexing, deep sample penetration, high signal-to-background, and sharp subcellular signal localization within fixed whole-mount zebrafish embryos, a standard model system for the study of vertebrate development.
For H&E and PAS staining, zebrafish were fixed in Bouins fixative for 2 weeks and embedded in paraffin.
For the histopathological examination, zebrafish were fixed with a mixture of 1.5% glutaraldehyde and 1.5% paraformaldehyde in 0.1 M sodium cacodylate (pH 7.4) buffer containing 0.001% CaCl2, they were subsequently rinsed in 0.1 M sodium cacodylate buffer containing 10% sucrose.
Zebrafish of 48 hpf were fixed overnight in Karnovsky's fixative and then processed for embedding in epon by the Microscopy and Imaging Laboratory core facility at the University of Michigan.
